电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

概要 ：聚焦辐射源否可通过转化生长因子-β（TGF-β）-介导的角质层-粘液转换 (EMT)来促进癌线粒体的蹂躏迁移。用于分之一2Gy(60)Coγ线紫外光称做人类心脏的6种癌线粒体，记录下来与EMT关的的波动，这有数分别利用显微镜系统设计，氨基酸；还有方法，免疫荧光系统设计，划痕检验和Transwell汤氏检验来通过观察并测定线粒体四组织形态，EMT标上，蹂躏迁移能够等。采用酶联免疫吸附法测定这些癌线粒体中TGF-β蛋白质总体，利用特别诱发剂SB431542来评估TGF-β信号通路在辐射源EMT中的作用。经过分之一为2Gy紫外光的癌线粒体中存在间叶线粒体的表达，与假紫外光四组相比其角质层标上减缓，间叶线粒体标上增加，同时其蹂躏重新分配能够增强，TGF-β蛋白质总体也提高。进一步发现由A549辐射源诱导的EMT可通过对TGF-β信号诱发频发翻盘。这些相比较TGF-β介导的EMT在辐射源诱导增强癌线粒体蹂躏重新分配能够中起着主导作用。

撰稿人： lizexiu